J.Cell Sci. 1979. 35: 267; Exp.Cell Res. 1980. 125: 305.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5.
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
86% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH and G6PD) analysis.
Karyology: 2n= 40, variability in the range between 62-68 chromosomes,
modal number of chromosomes 65-66, number of markers - 1-3, large meta- and
submetacentric chromosomes, the most cells have small metacentric
chromosomes (routine dye), number of polyploid cells 2.4%.
deficient in hypoxanthine phosphoribosyltransferase, resistant to 8 - azaguanine and 6 - thioguanine
somatic cell genetics