BWTG 3

Origin: mouse, hepatoma

            J.Cell Sci. 1979. 35: 267; Exp.Cell Res. 1980. 125: 305.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:5.

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     86% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis.

Karyology:   2n= 40, variability in the range between 62-68 chromosomes,

modal number of chromosomes 65-66, number of markers - 1-3, large meta- and

submetacentric chromosomes, the most cells have small metacentric

chromosomes (routine dye), number of polyploid cells 2.4%.

Other properties:

deficient in hypoxanthine phosphoribosyltransferase, resistant to 8 - azaguanine and 6 - thioguanine

Applications:  somatic cell genetics

Collections: SPBIC