African green monkey, kidney.
J.Immunol. 1963. 91: 416; Am.J.Public Health 1964. 54: 15-22.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3, optimal population density 1.5-2.0x105 cells/ml
cryoconservation -BME 70%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
72% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH and G6PD) analysis
Karyology: 2n= 60, variability in the range between 42-56 chromosomes,
modal number of chromosomes 53, number of markers-1, large submetacentric
chromosome with additional C band (differential dye), the cells have chromosomes
with secondary constriction and dicentrics, number of polyploid cells 6.0%.
virus susceptibility: SV40; polioviruses, vesicular stomatitis, arboviruses, parainfluenza,
herpes simplex, measles,
enteroviruses, influenza, RSV.
Susceptibility to the simian interferone.
Collections: ATCC CCL 26; ECACC 85011412; MWIIW.