BSC-1

Origin: African green monkey, kidney.

            J.Immunol. 1963. 91: 416; Am.J.Public Health 1964. 54: 15-22.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3, optimal population density 1.5-2.0x105 cells/ml

cryoconservation -BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 60, variability in the range between 42-56 chromosomes,

modal number of chromosomes 53, number of markers-1, large submetacentric

chromosome with additional C band (differential dye), the cells have chromosomes

with secondary constriction and dicentrics, number of polyploid cells 6.0%.

Plating efficiency: 12% (ATCC)

Other properties:

virus susceptibility: SV40; polioviruses, vesicular stomatitis, arboviruses, parainfluenza,

herpes simplex, measles, enteroviruses, influenza, RSV.

Susceptibility to the simian interferone.

Applications: virology.

Collections: ATCC CCL 26; ECACC 85011412; MWIIW.