BHK-21 clone 13

Origin: Syrian hamster, kidney

            Virology  1962. 16: 147-151; J.Natl.Cancer Inst. 1963. 30: 795-811.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -   FBS or BS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium (may add 30% BS), 5-10% DMSO,

1.0-1.5106 cells/ml in ampule

Viability after cryoconservation:     92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis.

Karyology:   2n= 44, variability in the range between 44-52 chromosomes, modal

number of chromosomes 49-50, number of markers - 1 large metacentric chromosome (routine dye)

Plating efficiency:    less than 1% (ATCC); 80% (ESCC)

Other properties:

virus susceptibility: pseudorabies, vaccinia, herpes simplex, reovirus 3; vesicular

stomatitis (Indiana), rubella, adenovirus 25, foot-and-mouth disease virus,

Coxsackie, rabies, arboviruses..

Applications:  virology, transformation, cell biology.

Collections: ATCC CCL 10; ECACC 85011433; SPBII; SPBIC, MWIEV.