BHK-21 (clone 13) v
Syrian hamster, kidney, subline of BHK-21 (clone 13)
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:5-1:6, optimal population density 0.5-0.7x105cells/ml
BME 70%, FBS 20%, glycerol 10%, 4.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
95 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=44, variability in the range between 35-45 chromosomes,
modal number of chromosomes 40, without markers (routine dye), number of polyploid cells 14%.
virus susceptibility: adenovirus 25; rabies; pseudorabies; foot and mouth disease;
herpes simplex; vesicular stomatitis;
reovirus 3; rubella; Coxsackie; vaccinia; arboviruses.
Collections: MWIIW; ESCC.