BHK-21 (clone 13) v

Origin: Syrian hamster, kidney, subline of BHK-21 (clone 13)

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:5-1:6, optimal population density  0.5-0.7x105cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 4.0-5.0106 cells/ml in ampule

Viability after cryoconservation:   95 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=44, variability in the range between 35-45 chromosomes,

modal number of chromosomes 40, without markers (routine dye), number of polyploid cells 14%.

Other properties:

virus susceptibility: adenovirus 25; rabies; pseudorabies; foot and mouth disease;

 herpes simplex; vesicular stomatitis; reovirus 3; rubella; Coxsackie; vaccinia; arboviruses.

Applications:  virology

Collections: MWIIW; ESCC.