BHK-21/ 2-17

Origin: Syrian hamster, kidney, subline BHK-21 clone 13.

            USSR Patent N 240289, 1986.

Morphology: epithelial-like

Mode of cultivation: monolayer, suspension

Conditions for cultivation: medium - 0.25% EMPH-d

serum -   BS 5%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:3-1:6

cryoconservation - growth medium 45%, BS 45%, 10% DMSO

or glycerol, 5.0-8.0106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, modal number of chromosomes 42.

Other properties:

virus susceptibility: foot and mouth disease.

Applications: virology.

Collections: MWIEV