BGM

Origin: African green monkey, kidney.

            Arch.Gesamte Virusforsch. 1970. 32: 389; Health Lab. Sci. 1974. 110: 275; Append.

Environ.Microbiol. 1986. 51: 790.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -   FBS 10%

other components -   NEAA 1% ( EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:8 - 1:20, optimal population density 5.0103 - 2.0104  cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 60, variability in the range between 58-68 chromosomes,

modal number of chromosomes 61-62, number of markers - 1-2, small submetacentric

chromosome with secondary constriction (routine dye)

Other properties:

virus susceptibility:  poliovirus 1, 2, 3; ECHO 3, 6, 7, 9, 11, 12, 27;

Coxsackie A9, B1, B2, B3; reovirus; rotavirus SA 11.

Applications:  virology, chlamidia growth substrate.

Collections: ECACC 90092601; MWIIW; SPBIC.