mouse C3H, smooth muscle-like cells from brain tumor induced in vivo by ethyl nitrosoethylurea.
J.Cell Biol. 1974. 61: 318-413; J.Biol.Chem. 1977. 252: 2143-2153.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 20%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 - 1:10, optimal population density 1.0-5.0õ104 cells/cm2
cryoconservation - growth medium, 8% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
95% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 40, variability in the range between 60-76 chromosomes,
modal number of chromosomes 64-67, number of polyploid cells 8%.
synthesis of adenylate and creatine phosphokinases, acetylcholine receptors.
Possess many properties characteristic of smooth muscle.
acetylcholine receptors study.
ATCC CRL 1443; ECACC 86093001; SPBIC.