BALB/3T3 clone A31

Origin:  mouse BALB/c, embryo.

            J.Cell Physiol. 1968. 72: 141-148; Virology 1969. 38: 174-202;

Science 1968. 162: 1024-1026; Exp.Cell Res. 1970. 59: 137.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or BME

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5 - 1:10, optimal population density 3.0103 - 2.0104 cells/cm2

cryoconservation - growth medium, 7.5% DMSO or glycerol, 1.0-3.0106 cells/ml in ampule

Viability after cryoconservation:    90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 55-84 chromosomes,

modal number of chromosomes 68-74, number of polyploid cells 3.0%.

Plating efficiency:    20% (SPBIC)

Other properties:

virus susceptibility: herpes simplex, vesicular stomatitis, coronavirus, SV 40, vaccinia, polyoma.

Contact inhibition of growth (by density 2.0-2.5105 cells/cm2).

Applications:  virology, replication, tumorigenicity.

Collections:   ATCC CCL 163; ECACC 86110401; MWIIW; SPBIC.