Chinese hamster, peritoneal cells, fibrosarcoma, derived from B14FAF28-G3.
Science 1961. 133: 1600; Tex.Rep.Biol. a Med. 1965. 23: 231.
Mode of cultivation:
Conditions for cultivation:
Mc Coy’s 5a
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:10
cryoconservation - growth medium, 5%DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
94% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) and immunofluorescent
Karyology: 2n= 22, variability in the range between 19-25 chromosomes,
modal number of chromosomes 22, pseudodiploid, a dicentric chromosome was
observed in some cells.
virus susceptibility: vesicular stomatitis (Indiana).
deficient in thymidine kinase, resistant to 5 - iododeoxyuridine
genetics, cell biology.
Collections: ATCC CCL 14.1; SPBIC.