B14-150

Origin:   Chinese hamster, peritoneal cells, fibrosarcoma, derived from B14FAF28-G3.

            Science 1961. 133: 1600; Tex.Rep.Biol. a Med. 1965. 23: 231.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - Mc Coy’s 5a

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:10

cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     94% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD)  and immunofluorescent analysis

Karyology:   2n= 22, variability in the range between 19-25 chromosomes,

modal number of chromosomes 22, pseudodiploid, a dicentric chromosome was observed in some cells.

Plating efficiency:    46% (SPBIC)

Other properties:

virus susceptibility: vesicular stomatitis (Indiana).

deficient in thymidine kinase, resistant to 5 - iododeoxyuridine

Applications:  genetics, cell biology.

Collections: ATCC CCL 14.1; SPBIC.