Origin: bovine, embryonic kidney.

            Submitted from Veterinary Research Institute, Brno, Czechia.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:5

cryoconservation - growth medium, 7.5%-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=60, variability in the range between 67-77 chromosomes,

modal number of chromosomes 73-74, number of polyploid cells 0.6%.

Other properties:

virus susceptibility: IBR, BVD.

Applications:  virology, veterinary science.

Collections: SPBIC