bovine, embryonic kidney.
Submitted from Veterinary Research Institute, Brno, Czechia.
Mode of cultivation:
Conditions for cultivation:
medium - EMEM or DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 - 1:5
cryoconservation - growth medium, 7.5%-10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
75% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=60, variability in the range between 67-77 chromosomes,
modal number of chromosomes 73-74,
number of polyploid cells 0.6%.
virus susceptibility: IBR, BVD.
virology, veterinary science.