Origin:hybrid cells - pig kidney SPEV TK- x equine lymphocytes .
Agricultural Biology 1990. N2: 182-187; USSR Patent N1417475,1988.
Morphology: epithelial- and lymphocyte-like
Mode of cultivation: monolayer, roller, suspension.
Conditions for cultivation: medium - EMEM, DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0,02%, split ratio 1:6-1:8.
cryoconservation - culture medium 50%, FBS 40%, DMSO 10%, 2-3x106 cells/ml in ampule
Viability after cryoconservation: 80-90%(0 passage, dye trypan blue)
Sterility: tests for bacteria,fungi and mycoplasma were negative.
Species : karyological analysis
virus susceptibility: equine herpes virus type 1 and 3, equine reovirus type 3.
Applications: virology, biotechnology