A4xC

 

Origin: hybrid cells - pig kidney x pig splenocytes.

Submitted by L.P.Dyakonov,L.N.Dudar,E.V.Maidji et al., 1994

J.Veterinary.1990 N 4:29-31; RF Patent N94026140/13, 1997.

Morphology: epithelial- and lymphocyte-like

Mode of cultivation: monolayer/suspension

Conditions for cultivation: medium - EMEM

serum - FBS 10%

subculture procedure - harvesting of

the cells in suspension is carried out by deposition, detachment

using trypsin 0,25% : EDTA 0,02% (1:9); split ratio 1:4-1:5.

cryoconservation - EMEM 50%, FBS 40%, DMSO 10%, 3-4x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria,fungi and mycoplasma were negative.

Species: karyological analysis

Karyology: 2n=38; variability in the range between

38-45 chromosomes; modal number of chromosomes 40.

Other properties:

virus susceptibility: coronavirus, transmissible gastroenteritis of swine, hog

cholera virus, encephalomyocarditis, equine herpes virus type 1.

Cells are able to virusinduced interferongenesis.

Applications: cell biotechnology, virology, biotechnology.

Collections: MWIEV