Origin:hybrid cells - pig kidney x pig splenocytes.
Submitted by L.P.Dyakonov,L.N.Dudar,E.V.Maidji et al., 1994
J.Veterinary.1990 N 4:29-31; RF Patent N94026140/13, 1997.
Morphology: epithelial- and lymphocyte-like
Mode of cultivation: monolayer/suspension
Conditions for cultivation: medium - EMEM
serum - FBS 10%
subculture procedure - harvesting of
the cells in suspension is carried out by deposition, detachment
using trypsin 0,25% : EDTA 0,02% (1:9); split ratio 1:4-1:5.
cryoconservation - EMEM 50%, FBS 40%, DMSO 10%, 3-4x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria,fungi and mycoplasma were negative.
Species: karyological analysis
Karyology: 2n=38; variability in the range between
38-45 chromosomes; modal number of chromosomes 40.
virus susceptibility: coronavirus, transmissible gastroenteritis of swine, hog
cholera virus, encephalomyocarditis, equine herpes virus type 1.
Cells are able to virusinduced interferongenesis.
Applications: cell biotechnology, virology, biotechnology.