mouse C3H/An, subcutaneous adipose connective tissue, derived from NCTC 929.
Proc.Natl.Acad.Sci. 1963. 50: 568; Nature 1964. 202: 1142; Am.J.Human Gen.
1974. 26: 273.
Mode of cultivation:
Conditions for cultivation:
medium - EMEM or DMEM
serum - FBS 10%
other components -
NEAA 1% (EMEM)
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:3 - 1:10, optimal population density 1.0-5.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
95% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 52-57 chromosomes,
modal number of chromosomes 54-55, number of markers - 1 (routine dye),
number of polyploid cells 1.8%.
deficient in hypoxanthine phosphoribosyltransferase, resistant to 8 - azaguanine and 6 - thioguanine
May be heterozygous for the ability to synthesize active inosinic acid phosphorylase.
metabolism, genetics of somatic cells.
Collections: ATCC CRL 6319; ECACC 84011426; SPBIC.