A-7

Origin: mouse CC57W, rhabdomyosarcoma induced in vivo by methylcholanthrene

            Submitted in Institute of Cytology RAS 1977.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - бле

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:5.

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     91% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH) and immunofluorescent analysis.

Karyology:   2n= 40, variability in the range between 46-63 chromosomes,

modal number of chromosomes 54-56, number of markers - 1, large metacentric

chromosomes (routine dye), 1-3 microchromosomes in the most cells, number of polyploid cells 28%.

Plating efficiency:    88%

Tumorigenicity:  tumorigenic in syngeneic mouse.

Applications:  tumorigenicity, cell biology.

Collections: SPBIC.