mouse CC57W, rhabdomyosarcoma induced in vivo by methylcholanthrene
Submitted in Institute of Cytology RAS 1977.
Mode of cultivation:
Conditions for cultivation:
medium - бле
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5.
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
91% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH) and immunofluorescent analysis.
Karyology: 2n= 40, variability in the range between 46-63 chromosomes,
modal number of chromosomes 54-56, number of markers - 1, large metacentric
chromosomes (routine dye),
1-3 microchromosomes in the most cells,
number of polyploid cells 28%.
tumorigenic in syngeneic mouse.
tumorigenicity, cell biology.