A-238

Origin: Chinese hamster, lung, clone of subline A-23 of cell line DON.

            Bioch.Genet. 1972. 7: 33; DAN Russ. 1982. 267. 6: 1496-1498; Cytology, Russ. 1985. 27. 4: 467-475.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:5

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 22, variability in the range between 30-48 chromosomes,

modal number of chromosomes 41-44, number of markers - 8 - in the most cells (differential dye).

Other properties:

deficient in thymidine kinase, resistant to BUdR.

Applications:  cell biology, genetics of somatic cells.

Collections:  SPBIC.