Chinese hamster, lung, clone of subline A-23 of cell line DON.
Bioch.Genet. 1972. 7: 33; DAN Russ. 1982. 267. 6: 1496-1498; Cytology, Russ.
1985. 27. 4: 467-475.
Mode of cultivation:
Conditions for cultivation:
medium - F10
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
98% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 22, variability in the range between 30-48 chromosomes,
modal number of chromosomes 41-44, number of markers - 8 - in the most cells (differential dye).
deficient in thymidine kinase, resistant to BUdR.
cell biology, genetics of somatic cells.