3T6 Swiss albino

Origin: Swiss mouse, embryo

            J. Cell Biol. 1963. 17: 299-313; Nature 1966. 212: 631-633.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5 - 1:20, optimal population density 5.0103 - 1.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 64-84 chromosomes,

modal number of chromosomes 70-72, some cells have large submetacentric

chromosome and microchromosomes ((routine dye).

Plating efficiency:    32% (SPBIC)

Other properties:

virus susceptibility: herpes simplex, vaccinia, vesicular  stomatitis (Indiana), pseudorabies.

Collagen and hyaluronic acid secretion.

Applications:  differentiation, proliferation study.

Collections: ATCC CCL 96; ECACC 86120801; SPBIC.