3T3B-SV40

Origin: mouse BALB/c, embryo, BALB/3T3 clone A31 transformed by SV40.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA  0.02% (1:3),

split ratio 1:10, optimal population density 2.0-4.0104  cells/cm2

cryoconservation - growth medium, 10% DMSO, 2.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, and isoenzymological (LDH, G6PD)  and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 66-73 chromosomes, modal number

 of chromosomes 70, 1-2 microchromosomes in 40% of cells.

Plating efficiency:    30%

Tumorigenicity:  non tumorigenic

Other properties:

T antigen in nucleus

Applications:  tumorigenicity, virology, cell biology.

Collections: SPBIC