3T3 Swiss albino

Origin: Swiss mouse, embryo. 

            J. Cell Biol. 1963. 17: 299-313.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:3 - 1:10, optimal population density 5.0103 - 1.0x104 cells/cm2

cryoconservation - growth medium, 5-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 65-73 chromosomes, modal number

of chromosomes 69-71, number of markers - 2-3, small acrocentric chromosomes

( routine dye), some cells have 1-2 microchromosomes, number of polyploid cells 1.2%.

Plating efficiency:    20% (ATCC)

Tumorigenicity:  non tumorigenic

Other properties:

virus susceptibility: herpes simplex, Sendai, vesic. stomatitis (Indiana), vaccinia.

Contact inhibition of growth.

Applications:  biochemistry, differentiation, virology, genetical transformation, tumorigenicity.

Collections: ATCC CCL 92; ECACC 85022108; SPBIC.