33 NIH TK-

Origin: mouse, embryo , subline 3T3 NIH

Cell 1979. 16: 63; 347.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:3 , optimal population density  1.0x105  cells/ml

cryoconservation - DMEM 70%, FBS 20%, glycerol 10%,  3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:   83 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n=40, variability in the range between 51-64 chromosomes, modal number

of chromosomes 59, number of polyploid cells 8.0%.

Other properties:

Deficient in thymidine kinase, resistant to 5-bromodeoxyuridine (25 mkg/ml)

Applications: virology, cytogenetics.

Collections: MWIIW