3T3-Swiss J2

Origin: Swiss mouse, embryo.

            Keratinocyte methods by I. and F. Walt. Cambridge University Press 1994. P.5-12.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:5, optimal population density 5.0103-1.0x104 cells/cm2

cryoconservation - growth medium, 8%DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=40, variability in the range between 70-80 chromosomes, modal number

 of chromosomes 74-76, number of markers - 1-3 metacentric chromosomes ( routine dye), number of polyploid cells 5.0%.

 

Other properties:

secretion of extracellular matrix protein for adhesion of keratinocytes and growth

factors for stimulation of keratinocyte proliferation.

Applications:   feeder for cultivation of epithelial cells.

Collections: SPBIC