Swiss mouse, embryo.
Keratinocyte methods by I. and F. Walt. Cambridge University Press 1994. P.5-12.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5, optimal population density 5.0õ103-1.0x104 cells/cm2
cryoconservation - growth medium, 8%DMSO, 1.0-2.0õ106 cells/ml in ampule
Viability after cryoconservation:
95% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=40, variability in the range between 70-80 chromosomes, modal number
of chromosomes 74-76,
number of markers -
1-3 metacentric chromosomes ( routine dye),
number of polyploid cells 5.0%.
secretion of extracellular matrix protein for adhesion of keratinocytes and growth
factors for stimulation of keratinocyte
feeder for cultivation of epithelial cells.