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Origin: rat, glioma induced by ethylnitrozourea.

            Submitted from Research Institute of Neurosurgery of the Ukrainian Ministry of Health, Kiev.

Morphology:  glial

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:5.

cryoconservation - growth medium, 10% DMSO, 1.0106 cells/ml in ampule

Viability after cryoconservation:     96% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 41-44 (first clone of cell population),

81-86 (second clone of cell population) chromosomes: , modal number of chromosomes 82-84,

 number of markers - 3 (differential dye).

Tumorigenicity:  tumorigenic

Applications:  neurobiology, tumorigenicity.

Collections: SPBIC