rat, glioma induced by ethylnitrozourea.
Submitted from Research Institute of Neurosurgery of the Ukrainian Ministry of
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5.
cryoconservation - growth medium, 10% DMSO,
1.0õ106 cells/ml in ampule
Viability after cryoconservation:
96% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 42, variability in the range between 41-44 (first clone of cell population),
81-86 (second clone of cell population) chromosomes: , modal number of chromosomes 82-84,
number of markers - 3 (differential dye).