2211

Origin: rat, glioma induced by N-methylnitrozourea.

            Exp.Oncol. (Russ) 1982. 2: 27.

Morphology:  glial

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:10.

cryoconservation - growth medium, 10% DMSO, 1.0106

cells/ml in ampule

Viability after cryoconservation:     97% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 78-85 chromosomes,

modal number of chromosomes 81-83, number of markers - 20 (differential dye).

Plating efficiency:    45%

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

secretion of protein S-100

Applications:  neurobiology, tumorigenicity.

Collections: SPBIC