rat, glioma induced by N-methylnitrozourea.
Exp.Oncol. (Russ) 1982. 2: 27.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:10.
cryoconservation - growth medium, 10% DMSO, 1.0õ106
cells/ml in ampule
Viability after cryoconservation:
97% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 42, variability in the range between 78-85 chromosomes,
modal number of chromosomes 81-83, number of markers - 20
tumorigenic in syngeneic animals
secretion of protein S-100