14.012.8.1

Origin: syrian hamster, fibroblasts transformed by herpes simplex virus.

Ann. Microbiol. (Paris). 1978, 129: 379.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density  1.0105 cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     91% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 44, variability in the range between 42-48 chromosomes,

modal number of chromosomes 44-45, number of markers - 1, one large submetacentric

chromosome (routine dye), number of polyploid cells 2.0%.

Other properties:

virus susceptibility: herpes viruses.

Infectivity DNA of herpes simplex virus in cells is higher then infectivity of intact virus.

Applications: virology.

Collections: MWIIW.