syrian hamster, fibroblasts transformed by herpes simplex virus.
Ann. Microbiol. (Paris).
1978, 129À: 379.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0õ105
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
91% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH and G6PD) analysis
Karyology: 2n= 44, variability in the range between 42-48 chromosomes,
modal number of chromosomes 44-45, number of markers - 1, one large submetacentric
chromosome (routine dye), number of polyploid cells 2.0%.
virus susceptibility: herpes viruses.
Infectivity DNA of herpes simplex virus in cells is higher then infectivity of intact virus.