L - 1 (Medicago sativa L.)

Origin: leaf

Morphology: light-yellow biomass

Mode of cultivation: liquid medium

Conditions for cultivation: medium - MS (kinetine 0.1mg/l, 2,4-D 1mg/l)

subculture procedure - change of medium on 15th day of cultivation

cryoconservation - growth medium, 7% DMSO

Viability after cryoconservation: 20-30% (dye phenosafranin)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis, biochemical determination of secondary substances

Karyology: 2n=16, variability in the range between 7-72 chromosomes,

modal number of chromosomes 18, aneuploid cells

Other properties:

superproducer of peroxidase

Applications: biotechnology

Collections : RCPC