IPHR DM 0.5 (Dioscorea deltoidea Wall.)

Origin: IPHR D1 after treatment by mutagene

Morphology: light-yellow biomass

Mode of cultivation: solid and liquid medium

Conditions for cultivation: modified medium - MS (kinetine 0.1mg/l, NAA 1mg/l)

subculture procedure - change of solid medium on the 30th day,

liquid medium on the 14th day of cultivation

cryoconservation - growth medium, 7% DMSO

Viability after cryoconcervation: 30%(dye phenosafranin)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis, biochemical determination of secondary substances

Karyology: 2n=20, variability in the range between 12-63 chromosomes,

two modal class: 2n and 3n, aneuploid cells

Other properties:

producer of diosgenin and furostanol glycosides

Applications: cell biology, biotechnology

Collections : RCPC