IPHR D1 (Dioscorea deltoidea Wall.)

Origin: root, callus was treatment by mutagene

Morphology: light-yellow biomass

Mode of cultivation: solid and liquid medium

Conditions for cultivation: modified medium - MS (kinetine 0.1mg/l, NAA 1mg/l)

subculture procedure -change of solid medium on the 30th day,

liquid medium on the 14th day of cultivation

cryoconservation- growth medium, 7% DMSO

Viability after cryoconservation: 30% after asparagine pretreatment (dye phenosafranin)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis, biochemical determination of secondary substances

Karyology: 2n=20, variability in the range between 8-49 chromosomes, two modal class 3n and 4n, aneuploid cells

Other properties:

producer of diosgenin and furostanol glycosides

Applications: cell biology, biotechnology

Collections : RCPC